diffraction limited wide field epifluorescence microscopy Search Results


ix73 inverted wide field epifluorescence microscope  (Evident Corporation)
99
Evident Corporation ix73 inverted wide field epifluorescence microscope
Ix73 Inverted Wide Field Epifluorescence Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ix73 inverted wide field epifluorescence microscope  (Olympus)
97
Olympus ix73 inverted wide field epifluorescence microscope
Ix73 Inverted Wide Field Epifluorescence Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence microscopes  (IonOptix)
90
IonOptix epifluorescence microscopes
Epifluorescence Microscopes, supplied by IonOptix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diffraction limited wide field epifluorescence microscopy  (Nikon)
99
Nikon diffraction limited wide field epifluorescence microscopy
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Diffraction Limited Wide Field Epifluorescence Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microscope nikon optiphot-2  (Nikon)
90
Nikon microscope nikon optiphot-2
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Microscope Nikon Optiphot 2, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence microscope zeiss epifluoresent  (Carl Zeiss)
90
Carl Zeiss epifluorescence microscope zeiss epifluoresent
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Epifluorescence Microscope Zeiss Epifluoresent, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescent, inverted microscope  (Evident Corporation)
90
Evident Corporation epifluorescent, inverted microscope
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Epifluorescent, Inverted Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence microscope  (Nikon)
90
Nikon epifluorescence microscope
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diaphot 300 inverted microscope  (Nikon)
90
Nikon diaphot 300 inverted microscope
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Diaphot 300 Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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optical ix-81 scope  (Evident Corporation)
90
Evident Corporation optical ix-81 scope
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Optical Ix 81 Scope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ccd digital camera hamamatsu c4742-95  (Hamamatsu)
90
Hamamatsu ccd digital camera hamamatsu c4742-95
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Ccd Digital Camera Hamamatsu C4742 95, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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conventional photomicroscope fitted for epifluorescence  (Kodak)
90
Kodak conventional photomicroscope fitted for epifluorescence
Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF <t>microscopy.</t> AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by <t>diffraction-limited</t> DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .
Conventional Photomicroscope Fitted For Epifluorescence, supplied by Kodak, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .

Journal: bioRxiv

Article Title: PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins

doi: 10.1101/2024.07.03.601615

Figure Lengend Snippet: Applications of PEPCy tags for imaging cell surface receptors. A . Schematic of membrane protein tracking experiment using TIRF microscopy. AlphaFold predicted structures of PEPCy tags. B . Normalized probability distributions of photons detected per trajectory from single particle analyses between PEPCy and HT targeted to their cognate dyes. (N = 1000-1300 single tracks per sample). C. Super-resolution microscopy of Intimin fused to PEPCy3 expressed on E.coli outer membrane. Schematic of the fusion is shown followed by diffraction-limited DIC and fluorscence images of the cells expressing the same. Maximum intensity projections (MIP) compared with a 2D reconstruction of single molecule localization data. Inset compares a cluster observable in the MIP that is resolved into two clusters in the super-resolved image. D . Time-lapse confocal microscopy of HeLa cells expressing PEPCy3-B2AR labeled using Cy3. Numbers on the left of each image denote the time in minutes. Isoproterenol was added at t = 0 and imaged were acquired every 30s for 1 hour. E . Schematic of simultaneous two color tracking of PEPCy3-B2AR and PEPCy5-B2AR-Ala on the same cell. F . Single molecule trajectories of PEPCy3 (left) and PEPCy5 (right) on a HEK cell, color coded by median trajectory speed. All scale bars in the figure unless mentioned otherwise are 5 µ m. A and E were created with BioRender.com .

Article Snippet: Prior to single molecule imaging PEPCy3-Cy3 signal was confirmed by diffraction limited wide-field epifluorescence microscopy, performed on a Nikon Eclipse Ti2 microscope equipped with a super apochromat objective (PlanApo, 100x, 1.45 N.A., oil immersion, Nikon) and scientific cMOS camera (Prime95B, Photometrix).

Techniques: Imaging, Membrane, Microscopy, Single Particle, Super-Resolution Microscopy, Expressing, Confocal Microscopy, Labeling